Fig. 4. Gata2 is induced in peripheral nerve precursors by ectopic expression of Phox2b and BMP24. Chick embryos were infected at E2 with RCAS-Phox2b and RCAS-BMP4 and analysed at E8 for the presence of ectopic neurons in the brachial nerve. In response to Phox2b and BMP24 neurons were generated that express noradrenergic properties: Th (A,C) and Gata2 (B,D). These cells also coexpress neuronal characteristics (Stanke et al., 1999; Howard et al., 2000).