Fig. 6. The expression of a dominant-negative variant of Gata2 results in a reduction of Th, Scg10, Dbh and Phox2b expression. Chick embryos were infected unilaterally at E2 with either RCAS-dnGata2 or RCAS-engrailed and RCAS-VP16-Gata2 as controls and analysed at E8 for the expression of Th (A), Scg10 (B), Dbh (C) and Phox2b (D) in infected sympathetic ganglia. The areas of Th-, Scg10-, Dbh- or Phox2b-expressing cells were quantified on alternate sections, both on the infected and the contralateral uninfected side. For the RCAS-engrailed and RCAS-VP16-Gata2 controls, only data of the infected ganglia are shown for simplicity; the data of the uninfected side are identical. The data represent the mean±s.e.m. of at least nine embryos. (A) ^* significantly different from engrailed-infected side, VP16-Gata2-infected side and dnGata2-uninfected side (P<0.001, P<0.005, P<0.005, respectively). (B) ^* significantly different from engrailed-infected side, VP16-Gata2-infected side and dnGata2-uninfected side (P<0,005, P<0,01, P<0.01, respectively). (C) ^* significantly different from dnGata2-uninfected side (P<0.005). (D) ^* significantly different from dnGata2-uninfected side (P<0.01) (all Student's t-tests).