Fig. 9. Gata2 expression in peripheral nerve precursors results in the generation of neurons that display mostly a non-autonomic phenotype. Chick embryos were infected by RCAS-Gata2 at E2 and analysed at E8 for the expression of ectopic neurons in the brachial nerve. Whereas a large number of Scg10-positive cells was detected (A, overview; B, enlargement of the region of the nerve), only few cells expressing autonomic marker genes (C, Phox2b; E, Cash1) or noradrenergic markers (D, Th) were present. This is confirmed by double in-situ hybridisations for Scg10 (red, F) and Th (blue, G). Please note the low number of cells that coexpress Scg10 and Th (arrow) and of Th⁺, Scg10^– cells (arrowheads).