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Fig. 3. Amn is not required for cell proliferation or differentiation in fetal kidney and intestine. Cryosections from E16.5 Amn-/-{leftrightarrow}+/+ chimeras. (A,C) Two kidney sections and (B,D) two intestine sections stained with X-gal to identify wild-type (blue cells indicated by blue arrows) and Amn-/- cells (pink cells indicated by red arrows). (A) Amn-/- cells colonize the kidney and contribute to glomeruli (G) and tubules. (B) Amn-/- cells colonize the intestinal villi (V). (C) Section of a chimeric kidney containing ~100% Amn-/- cells with normal gross morphology, including properly formed glomeruli (G) and tubules. (D) A section of chimeric intestine containing ~100% Amn-/- cells with normal morphology and well-formed villi (V). (E) Chimeric kidney section stained with LTL. (Inset) Kidney cryosection from an E16.5 Amn+/-{leftrightarrow}+/+ chimera stained with LTL. (F) X-gal staining of section adjacent to (E) identifies Amn-/- cells in properly differentiated proximal tubules (PT). (Inset) X-gal staining of kidney cryosection from an E16.5 Amn+/-{leftrightarrow}+/+ chimera.