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Fig. 5. Cubn is not localized to the apical cell surface in Amn-deficient KPT. Immunohistochemical analyses of serial paraffin sections (A,D,G) from an adult chimeric kidney with high contribution of Amn-/- cells. Two sets of serial high magnification views are shown in B,E,H and C,F,I. (A-C) Cubn, (D-F) Amn and (G-I) megalin antibody staining of wild-type KPT (blue asterisks; WT) and Amn-deficient KPT (red asterisks; mut). A chimeric tubule (green asterisks; ch), which contains both wild-type and Amn-/- cells, is designated as well as a distal tubule (yellow asterisks; DT). Of significance, Cubn does not localize to the apical surface in Amn-deficient KPT but is observed in the cytoplasm (B,C).