Fig. 6. Prolonged mis-expression of Dup is sufficient to induce re-replication and cell death in the ovary. Follicle cells labeled with Toto-3 (blue) and BrdU (red). (A) Stage 6 wild-type egg chamber. (B) FL-Dup expression in stage 5 and 6 follicle cells results in enlarged nuclei, some of which are actively replicating (two indicated by arrows), while others are pycnotic (arrowhead). The punctate BrdU incorporation is late replication of heterochromatin. (C) Myc:Dup 10(A) expression also results in enlarged nuclei that incorporate BrdU (arrow), and pycnotic nuclei (arrowhead). Images are composites of confocal sections. Scale bar: 10 µm.