Fig. 2. GLD-1-dependent repression of GLP-1/Notch results in underproliferation of germ cells in ATX-2-depleted gonads. (A) The mean number of phosphorylated-H3-positive, mitotic nuclei per gonad arm (n=number of gonad arms)±mean deviation, was determined for animals with the indicated genotypes. The fem-3(gf) and fem-1 mutations masculinize and feminize the germline, respectively. Depletion of GLD-1 restored the number of mitoses in gld-1;atx-2(RNAi) gonads to the wild-type level. (B-G) Magnified images of the distal gonad after staining for GLP-1 (B-D) or GLD-1 (E-G). Abnormally high levels of GLD-1 are present in the distalmost part of masculinized atx-2(RNAi) gonads (F), but not in many feminized, fem-1;atx-2(RNAi) gonads as shown here (G). Scale bars: 50 µm.