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Fig. 4. Germ cells fail to enter pachytene in gld-2;atx-2(RNAi) gonads. Dissected gonads after staining for HIM-3 or with DAPI; insets show magnified views of nuclei from regions marked with arrowheads. Photographs in A and B were taken with identical exposures, as were photographs in D and F. Pachytene chromosomes in A-D have a characteristic thread-like appearance. The chromosomes of gld-2;atx-2(RNAi) germ cells did not resemble normal pachytene nuclei in morphology when stained with DAPI (E) or in HIM-3 localization (F). Approximately half of the gld-2;atx-2(RNAi) gonads analyzed showed inappropriate mitotic proliferation in the proximal germline (region indicated by bent arrow in E,F). Scale bars: 50 µm.