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Fig. 5. The yolk receptor RME-2 is expressed abnormally in ATX-2-depleted gonads. (A-D) Gonads from animals with the indicated genotypes stained for RME-2. (E) Distal region of a wild-type gonad stained for MEX-3. (F) Distal region of a mex-3(RNAi) gonad showing inappropriate expression of RME-2. (G) Composite image showing the distal regions of gonads from animals with indicated genotypes after in situ hybridization for rme-2 mRNA. Only a low level of hybridization is present in the distalmost region (brackets) of the wild-type or mex-3 gonad. (H,I) Central region of gonad from a glp-1(gf);mex-3(RNAi) animal after staining for RME-2 (H) and DAPI (I). Scale bars: 50 µm.