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Fig. 4. Increased apoptosis detected in Pcm mutant spleens. TUNEL analysis at E15.5 (A) and E16.5 (B) indicates a significant increase in TUNEL-positive foci in Pcm mutant spleens, higher in homozygotes. Images represent merged composites of TUNEL (fluorescein) and DAPI-counterstained sections, original magnification 200x. (C) Immunohistochemistry for F4/80 at E15.5 reveals similar distribution of positive cells within spleens across all genotypes. Original magnification, 400x. (D) Immunohistochemistry for Wt1 at E15.5 demonstrates widespread stromal cell staining within the spleen. Original magnification, 400x.