Fig. 3. Expression of Zfp36l2 transcripts in tissues from mutant mice. (A) Total RNA was extracted from tissues of wild-type (WT) and N-Zfp36l2 mutant mice and subjected to northern blot analysis. Each lane contained 10 µg of total RNA, hybridized with Zfp36l2 exon 2 and Gapd ³²P-labeled probes, as indicated by the arrows. Note the decreased size and expression levels of the mutant transcript. (B) The Zfp36l2 transcripts from the WT and N-Zfp36l2 mutant mice were quantified with a PhosphorImager, and normalized by Gapd mRNA levels. The normalized values were plotted for each tissue. (C) The normalized Zfp36l2 transcript levels in tissues from the N-Zfp36l2 mutant mice were expressed as percentages of WT transcript levels. The data for bone marrow-derived macrophages (BMMac) were taken from a separate experiment.