Fig. 4. Changes in nuclear Lamins are associated with increased levels of active caspase. Salivary glands from wild-type Canton S were dissected and stained with antibodies against: nuclear Lamin DmO (red) and active Caspase-3/Drice (green) (top panels); and nuclear Lamin DmO (red) and cleaved nuclear Lamin (green) (bottom panels). Nuclear Lamin DmO is present 8 and 10 hours apf, but decreases by 12 hours apf. At this stage, active Caspase-3/Drice and cleaved nuclear Lamin are present in the cytoplasm; levels of these increase by 14 hours apf.