Fig. 6. Animals with mutations in ßFTZ-F1, E93, BR-C and E74A have altered levels and localization of structural proteins. Salivary glands were dissected from mutants 24 hours apf, and analyzed for the levels and localization of: -Tubulin (red) and -Spectrin (green) (top panels); filamentous Actin (red) (middle panels); and nuclear Lamin DmO (red) and Croquemort (green) (lower panels). The -Tubulin network is present in the salivary glands of all of these mutants, but it appears to be at slightly lower levels in the cytoplasm of E93 mutants. -Tubulin appears to be absent near the cortex in BR-C and E74A mutant animals, given the lack of overlap with -Spectrin staining. -Spectrin is present along the cortex in ßFTZ-F1, E93 and BR-C, and in fragments in the cytoplasm of E74A mutant salivary glands. Filamentous Actin is aggregated in clumps in the cytosol, and is expressed along the cortical region of salivary gland cells in ßFTZ-F1, E93, BR-C and E74A mutant animals, although differences clearly exist in the localization of this protein in the different mutants. Nuclear Lamin DmO is present and is evenly expressed in association with the nuclear membrane in ßFTZ-F1 mutant salivary gland cells, is expressed at a lower level with punctate spots in E93 and BR-C mutant salivary glands, and is difficult to detect in E74A mutant salivary gland cells. Croquemort is not detectable in ßFTZ-F1 mutant salivary glands, is expressed at a lower level in E93 and BR-C salivary glands, and is highly expressed in E74A mutant salivary gland cells.