Fig. 7. Mutations in ßFTZ-F1, E93, BR-C and E74A impact caspase activation during autophagic cell death. Salivary glands were dissected from mutants 24 hours apf, and either analyzed for abundance and localization of nuclear Lamin DmO (red) and active Caspase-3/Drice (green) (top panels), or nuclear Lamin DmO (red) and cleaved nuclear Lamin (green) (bottom panels). Active Caspase-3/Drice and cleaved nuclear Lamins are absent in ßFTZ-F1 salivary glands, are present at extremely low levels in E93 and BR-C salivary gland cells, and are expressed at elevated levels in E74A mutant salivary gland cells.