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Fig. 5. Morphology and neurosphere cultures from Gli3 null brains. (A) Dorsal morphology of wild-type (left) and Gli3-/- (right) dissected brains at E15.5. Anterior is to the top. (B,C,E,F) Phase contrast images of representative primary cultures of wild-type (B,E) and Gli3-/- (C,F) animals at E15.5 (B,C) and E18.5 (E,F). Insets (E,F) correspond to TuJ1+ immunostaining of primary cultures. (D) RT-PCR analyses of E15.5 first passage nsps representing the rare survivors. Hprt was used as a loading control. RT-PCR analyses at E18.5 were not possible due to the lack of nsps.