Fig. 11. Application of ephrinB2-Fc and EphB4-Fc on the allantois. (A-I) Ephrin-B2 application. (J-L) EphB4 application. Still images of time-lapse video-microscopy taken at the indicated time points. Arteries (A) and veins (V) in the application area are indicated. Note increased venous branching (C,D, arrows), and rapid formation of arterial-venous shunts after ephrinB2-Fc application (arrowheads in F-I). Note enlargement of the artery in EphB4-Fc treated embryos (J-L) as well as formation of arterial-venous shunts (arrowhead, L). Scale bar: 830 µm.