Fig. 5. Posterior markers are normal in sec5^E13 oocytes. (A) Wild-type and (B) sec5^E13 oocytes labeled with anti-Oskar (green). (C) Par-1 (green) localizes properly in sec5^E13 clones (arrow). Phalloidin (red) and the oocyte nucleus(^*) are shown. (D) A Kinesin-ßgal fusion (green) localizes to the posterior end of the sec5^E13 oocyte. In wild-type (E,F) and sec5^E13 (G,H) egg chambers, Dynein Heavy Chain (green) accumulates in the oocyte between stages 1-6 (E,G) and subsequently (F,H) at its posterior end (arrow). Scale bars: 20 µm.