Fig. 6. The microtubule cytoskeleton is oriented correctly in sec5^E13 oocytes, although the oocyte nucleus is displaced from the cortex. (A) Wild-type and (B) sec5^E13 stage 7 egg chambers expressing Nod-GFP (green) under the control of nanos-Gal4 and stained with phalloidin (red). (C-F) Stage 10 egg chambers, stained as above. In sec5^E13 oocytes, Nod-GFP properly concentrates anteriorly (D). The oocyte nucleus (^*) is also often at the dorsal anterior corner. However, the overexpression of Nod-GFP in the germline enhances the sec5^E13 phenotype (E,F). (G-H) Single z sections of stage 10 egg chambers labeled with phalloidin (red) and nuclei with Hoechst (blue), and the position of the oocyte nucleus shown with an arrow. (I,J) The full z stack of G and H were rotated in three dimensions and the nurse cells optically removed to reveal the close apposition of the nucleus to the surface in wild type (I), but not in the sec5^E13 chamber (J). (K-O) Egg chambers stained for Bicaudal-D (green), phalloidin (red) and with Hoechst (nuclei, blue), with genotypes indicated. (M-O) Bicaudal-D (arrows) remains associated with the nucleus (^*) whether in wild type or sec5^E13 oocytes. Scale bars: 20 µm.