Fig. 6. Comparison of developmental changes in the levels of discoidin 1 gene expression in Dd-STAb+ and Dd-STATb-clones. The kinetics and extent of discoidin 1 mRNA accumulation were determined for two random integrant, Dd-STATb+ clones (B15 and B16, Fig. 3B) and two disruptant, Dd-STATb-clones (B8 and B9, Fig. 3B). Cells were grown to a density of 2x10⁶ cells/ml and subjected to development in shaken suspension in KK2 for the indicated time periods.