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Fig. 2. Analysis of protein and RNA levels in adult testes. (A) Western blot confirming that AR protein levels are reduced in testes of Arflox(ex1-neo)/Y and Arflox(ex1-neo)/Y; Amh-cre males. (B) Northern blot showing decrease in Prm1 and Pem mRNA expression in testes of Arflox(ex1-neo)/Y and Arflox(ex1-neo)/Y; Amh-cre males. (C) Quantification of mRNA levels from B. Values with the same superscript are significantly different from one another (a,c,d,f, P<0.05; b,e, P<0.001). n=4 for all groups.