Fig. 4. Evaluation of somatic and germ cell protein expression. (A-F) MSY4 expression in pachytene spermatocytes and round spermatids is unaffected in Ar mutant males. Elongated spermatids are reduced in (B,E) and absent from (C,F) the tubules of Ar^{flox(ex1-neo)/Y} and Ar^{flox(ex1-neo)/Y}; Amh-cre, respectively. (G-I) PRM2-positive elongated spermatids are decreased in Ar^{flox(ex1-neo)/Y} males (H) and absent from Ar^{flox(ex1-neo)/Y}; Amh-cre males (I) at time of spermiation. (J-L) AR protein is expressed in somatic cells of the testis. AR is present in Sertoli cells of Ar^{flox(ex1-neo)/Y} males (K, blue arrowheads).