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Fig. 1. Flow cytometric analysis of Hoechst 33342 fluorescence. (A) C57BL/6 adult murine testicular cells. (B) Definition of the five main subpopulations and respective percentages of total testicular viable cells: 1 (1%), 2 (2.4%), 3 (8%), 4 (7%), 5 (70%). (C,D) Comparison with two infertile murine models: W/Wv mutant (C) and cryptorchid C57BL/6 male (D). (E,F) Identification of somatic interstitial cells: interstitial cell-enriched fraction (E) compared with the fraction enriched in seminiferous tubules (F). Vertical axis shows blue (Ho) fluorescence, horizontal axis red (Ho/PI) fluorescence.