Fig. 3. Analysis of the SP population for the expression of
6-integrin and Stra8 germinal stem cell markers. Ho
fluorescence flow cytometric analyses of
6-integrin-positive
cell fraction (A) and
6-integrin-negative cell fraction (B)
after immunomagnetic sorting. (C) RT-PCR analysis of Stra8 gene
expression (35 cycles) on W/Wv, and sorted normal
populations: SP cells (SP), tetraploid population (4n), upper diploid
population (2n) and haploid population (n).