Fig. 3. Extra growth produced by brk mutant clones. (A,B) brk^– clones (A, yellow; B, forked³⁶) in the anterior (A) and posterior (B) compartments. The clones do not produce pattern duplications but do produce additional tissue. (C) A disc doubly labelled arm-lacZ (green) and BrdU (red) with a brk^– clone (arrow) showing greater BrdU incorporation than surrounding cells. (D,E) Disc of genotype nub-Gal4>UAS-dad stained for wg (red) containing two brk^– clones (arrows) marked by the loss of GFP (green). The two clones appear in the wing pouch (which is delineated by the internal wg ring in E) and overgrow, even though they have originated in the wing pouch where there is virtually no Dpp activity.