Fig. 9. Brk downregulates bantam. (A,B) Wing disc with normal brk activity stained for PH3 and the bantam sensor: the brighter green colour corresponds to low bantam expression (see main text). The arrow indicates a characteristic zone of low bantam levels located in the brk domain. The distribution of the PH3 dots in the disc is uniform, indicating the uniform cell proliferation levels in the disc. (C,D) Disc of nub-Gal4>UAS-tkv^QD genotype showing more PH3 staining in the lateral region, which is associated with partial loss of bantam. (E,F) A brk^– clone showing greater bantam activity (arrows), as indicated by the reduction in the level of green staining.