Fig. 8. kni upregulation by ash2^I1 is independent of sal-C downregulation. (A) Overexpression of sal-C from UAS-sal^64d using a nubbin-Gal4 driver results in loss of L2 and L5 and severe reduction of wing size. (B) Wing imaginal disc showing ectopic expression of salm throughout the wing pouch. (C) ash2^I1 homozygous clones are devoid of GFP. (D) kni expression is also upregulated in ash2^I1 mutant clones even, when salm is overexpressed. (E) Merged images. Scale bars: 20 µm.