Fig. 4. Phenotypes of AtBRM-silenced plants (atbrm). (A) Levels of AtBRM mRNA in different kanamycin-resistant transgenic lines transformed with pART27-AtBRM-RNAi. Total RNA was isolated from inflorescence apices, blotted and hybridized with an AtBRM probe. The blot was stripped and rehybridized with an 18S ribosomal probe (18S) as a loading control. (B) Levels of AtBRM protein in AtBRM-silenced plants. 20 µg of nuclear protein were subjected to western blotting using -AtBRMb antiserum. (C) Wild-type (Columbia) and atbrm (line 29.1) plants at 23 days of growth in LD. (D) Higher magnification of one atbrm plant shown in C. Scale bar: 5 mm. (E) Rosette leaves from wild-type and atbrm (lines 10.1 and 29.1) plants grown in LD. Scale bar: 1 cm. (F) Cauline leaves from wild-type and atbrm plants (lines 10.1 and 29.1) grown in LD. Scale bar: 1 cm. (G) Inflorescences from wild type and atbrm (line 29.1) plants grown in SDs. Scale bar: 1 mm. (H) Cross section of curled rosette leave from a atbrm plant (line 29.1). Scale bar: 1 mm.