Fig. 7. Early flowering phenotype of atbrm plants (line 29.1). (A) Wild-type and atbrm plants at 40 days growth under SD conditions. (B-E) RNA was isolated from total seedlings, collected 9 hours after dawn, at 10, 13 or 16 days of growth under SD conditions (10 hours light/14 hours dark). RNA blots were hybridized with a FLC- (B) or with a SOC1 (E)-specific probe and reprobed with 18S ribosomal DNA (18S) as a loading control. Levels of CO (C) and FT (D) mRNA were determined by semi-quantitative RT-PCR. Levels of the ubiquitin mRNA were also tested by RT-PCR as control.