Fig. 6. MO3 causes a phenotype similar to that caused by MO1. (A-D) Embryos injected with increasing concentrations of an alternative activin B antisense morpholino oligonucleotide termed MO3 (B-D) show a similar phenotype to that observed with MO1, although MO3 is effective at lower concentrations (compare C with Fig. 3B). (E-J) Expression of Goosecoid and Chordin is reduced by both MO1 and MO3, and expression of Xvent1 is elevated. For MO1, mMO1 was used as a control, and for MO3, MO2 was used as a control. Embryos were analysed at the indicated stages. In this experiment, inhibition of Goosecoid expression by MO3 was most marked at stage 11 (H).