Fig. 10. Cdc25B overexpression, but not PDE3A overexpression, overcomes the meiotic block induced by PTPN13 siRNA. Ten nanograms of siRNA-1, scrambled siRNA-1 or vehicle were injected into Xenopus oocytes. After 12 hours, oocytes were stimulated with (A) 500 nM progesterone, or injected with (B) 1 ng of Cdc25B mRNA or (C) 20 ng of PDE3A mRNA to induce oocyte maturation, and resumption of meiosis was scored. Results shown are representative of the two experiments performed.