Fig. 5. Effects of C-PTPN13 injection on progesterone-inducing Xenopus oocyte maturation. (A-C) Twenty nanograms of HA-tagged C-PTPN13 mRNA (in 20 nl H₂O) or 20 nl of H₂O were injected into 30 Xenopus oocytes and 12 hours later oocytes were stimulated with the indicated concentration of progesterone. Resumption of meiosis was scored at the indicated time by the appearance of a white spot on the animal pole of the oocyte. (D) Different amounts (as indicated) of HA-tagged C-PTPN13 mRNA were injected into Xenopus oocytes and 12 h later oocytes were stimulated with 500 nM progesterone. Results shown are representative of the two independent experiments performed.