Fig. 8. Effects of PTPN13 siRNA injection on Xenopus oocyte maturation. Ten nanograms of specific PTPN13 siRNAs (A) or scrambled siRNAs (B), or indicated amounts of PTPN13 siRNA-1 (C) or scrambled siRNA-1 (D) were injected into Xenopus oocytes. After 12 hours, oocytes were collected and total RNA was isolated. Two micrograms of total RNA was used for semiquantitative RT-PCR. After 12 hours, oocytes were stimulated by 500 nM progesterone for 10 hours (A,B) or the indicated numbers of hours (C,D) and resumption of meiosis was scored. These experiments were repeated at least five times and representative results are shown.