Fig. 9. Effects of PTPN13 siRNA injection on progesterone-dependent changes in signaling molecules in Xenopus oocytes. Ten nanograms of PTPN13 siRNA-1 or scrambled siRNA-1 were injected into Xenopus oocytes. After 12 hours, oocytes were stimulated with 500 nM progesterone for 8 hours. The oocyte lysates were subjected to 8% SDS-PAGE, and the expression and phosphorylation of each protein were detected by western blotting analysis.