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Fig. 2. citron is expressed maternally and in proliferating tissues during Drosophila development. Whole-mount in situ hybridisation with a Drosophila citron DIG-labelled RNA probe. (A) A stage 10A oocyte showing citron transcripts in the nurse cells. (B) Uniform distribution of citron transcripts in a blastoderm embryo. Maternally provided citron mRNA has degraded by stage 9, compare wild-type (C) and homozygous Df(3)iro-2 mutant (D) embryos. (E-H) Zygotic tissue-specific expression of citron in the proliferating CNS (arrow) and PNS (arrowhead) starts during germ-band retraction. Anterior is to the left, dorsal to the top.