Fig. 8. Citron acts as a positive factor in the Pebble-Rho signalling pathway. Images of whole adult wings. (A) An en-GAL4>UAS-pbl^RNAi wing in a wild-type background. (B) An en-GAL4>UAS-pbl^RNAi wing heterozygous for cit¹ shows significant enhancement of the pbl^RNAi phenotype. (C) An en-GAL4>UAS-pbl^RNAi wing heterozygous for rok² does not appear be significantly modified. (D) Co-expression of a gain-of-function allele of citron (UAS-cit²) and en-GAL4>UAS-pbl^RNAi slightly rescues the pbl^RNAi phenotype. (E) The number of multiple haired wing cells in a defined region (posterior to L5, framed in Fig. 7A) was scored for each genotype shown in Fig. 7 and Fig. 8 in order to quantitate the effect. Multiple haired cells were scored in 14 adult wings for each genotype. Bars represent the percentage of multihaired cells in each genotype. Error bars represent one standard deviation. Statistical significance of the difference between two pairs of genotypes was determined using a ² 2x2 contingency test (P<0.001). Significant enhancement of the en-GAL4>UAS-pbl^RNAi phenotype was seen when flies were heterozygous for RhoA^72R, cit¹ or Df(3)iro-2 alleles.