Fig. 6. Maternal FoxH1, XTcf3 and VegT regulate Xnr5 and 6. (A) The relative expression level of Xnr5 and 6 in wild-type, FoxH1-depleted, XTcf3-depleted and FoxH1/XTcf3-depleted embryos at the stages shown assayed by real-time RT-PCR and normalized to ODC. Both FoxH1 and XTcf3 depletion enhances the expression of Xnr5 and 6 compared to controls, but the double depletion does not have an additive effect. (B) The relative expression level of Xnr3 and 5 in wild-type, FoxH1-depleted, VegT-depleted and FoxH1/VegT-depleted embryos at the stages shown assayed by real-time RT-PCR and normalized to ODC. Xnr5 over-expression in FoxH1-depleted embryos is dependent on maternal VegT. In comparison, Xnr3 expression is not dependent on VegT.