Fig. 1. Synergistic effects of Wg and Frizzled on LEF-dependent transcription. S2 cells were transfected with indicated plasmids and plasmids as described in the Materials and methods. Fold-activation values were measured relative to the levels of luciferase activity in cells transfected with an empty vector and normalized by Renilla luciferase activities. All experiments were carried out in triplicate and error bars represent standard deviation.