Fig. 7. Strong synergy between human FZ5 and LRP6 in Wnt/ß-catenin signaling by fusing the intracellular domain of LRP6 to human FZ5. (A) Schematic representation of human FZ5, LRP6 and the HFz5-LRP6 chimera. The intracellular domain of LRP6 was fused to the C terminus of human FZ5 to form HFz5-LRP6C. The C terminus of HFz5-LRP6C was fused with GFP. (B) Hyperactivity of HFz5-LRP6C. S2 cells were treated with control or Dsh-dsRNA and transfected with the indicated plasmids. As control, Arg263, Leu443 or Lys525 of human FZ5 was mutated in HFz5-LRP6C. Membrane expressions of HFz5-LRP6C and its mutants were determined by the membrane biotinylation assay (insert).