Fig. 5. Rescue of neuronal lamination is induced by reelin. (A,B) Reeler hippocampus co-cultured next to rat hippocampus; the dotted line represents the border between the two cultures. Neuronal somata stained for Neurotrace (green); counterstained for reelin (anti-reelin G10; red). A compact neuronal layer (arrows) has only formed in those portions of the reeler culture that are juxtapositioned to reelin-synthesizing cells in the outer molecular layer of the rat culture. (C) Reeler hippocampal culture co-cultured next to a rat olfactory bulb (OB) culture. The border between the two cultures is marked by a dashed line. A dense neuronal layer (arrow) in the reeler culture has formed near the border of the rat olfactory bulb culture, containing numerous reelin-synthesizing mitral cells (red). (D) A compact neuronal layer in the reeler dentate gyrus next to reelin-synthesizing neurons in a rat co-culture fails to form when the two cultures were incubated in the presence of the reelin-blocking CR-50 antibody. Scale bars: 175 µm in A,B,C; 150 µm in D.