Fig. 3. Pioneer and follower L1 glial cells. (A) Schematic representation of a wing. Boxes show regions of interest on L1 (B-D) that have been analysed in different repo-ncGFP animals. (B,C) Individually labelled (repo-ncGFP MARCM clones) follower cells. (B) Distal glial cell at 17 hours APF. (C) Time-lapse sequence (17-19 hours APF) on two glial cells. (D) Time-lapse sequence (17-19 hours APF) on the whole glial population at the front of migration (see also Movie 3 in supplementary material). Pioneer glial cells display more numerous and elaborate filopodia (arrowheads) when compared with follower cells, which are more distal (see asterisk). Scale bars: 30 µm (B-D).