Fig. 6. Aged T
1-Cre;TrkAneo/neo mice have increased
levels of peritoneal B1 cells. Twelve- to eighteen-month-old mutant
(n=6) and control (n=6) littermates were sacrificed and
peritoneal cells were collected as previously described (32). Similar numbers
of cells were recovered from both groups. Cells were stained for FACS analysis
with anti-B220, -IgM, -CD5, IgD and -Mac-1 antibodies in triple staining
combinations. Three staining combinations are shown (B220/IgM; Mac-1/IgD;
B220/CD5). The B1 cell population is indicated by a box. Percentages of
peritoneal B2 cells
(B220+/IgMlow/IgDhigh/Mac-1–)
and macrophages (B220–/Mac-1+) were not different
in mutant mice compared with controls.