Fig. 10. Nkx6 misexpression affects both motoneurons and interneurons in flies. (A) Wild-type fly embryo with normal numbers of aCC, pCC, and RP2 (arrow) neurons (n=48). (B) In sca>Nkx6 (fly gene) embryos RP2 is duplicated (arrow) in 75% of hemisegments (n=34). (C) Wild-type embryos have 5.0±0.14 U motoneurons (asterisks; n=48) and 10.0±0.73 EL interneurons (circle; n=46). (D) In sca>Nkx6 embryos both types of neurons are reduced (Us, 2.5±1.4, n=34; ELs, 5±3.4, n=34). (E) In wild types there are 3.8±0.48 RP1,3,4,5 motoneurons (circle; n=80) and 2.8±0.37 EW interneurons (arrowhead; n=25). (F) In sca>Nkx6 (fly gene overexpression, OE) RP1,3,4,5 are normal (circle; 3.6±0.65; n=73) and EWs are reduced (arrowhead; 1.0±1.2; n=30). In some cases, as shown here, an EW-like cell ectopically expresses pMAD, indicating an interneuron-to-motorneuron fate change; these cells were not scored as EWs. (G) In sca>nkx6.1 (fish gene) RP1,3,4,5 numbers are normal (circle, 3.5±0.66; n=55). (H) Wild-type embryos have one medial lateral motoneuron (ML) per hemisegment (asterisk; 80±0; n=80) and 4.7±0.54 HB9-, Islet-, pMAD-positive lateral cluster motoneurons (LC; circle; n=50). (I) sca>Nkx6 (fly gene) embryos have one ML motoneuron (asterisk; 94% of hemisegments; n=44) and 8.5±2.3 LC motoneurons (n=80). (J) sca>nkx6.1 (fish gene) also have one ML motoneuron (asterisk, 89.4% of hemisegments; n=57) and 8.0±1.89 LC motoneurons; (n=60). (K) Schematic depicting average wild-type number of assayed motoneurons and interneurons. (L) Schematic depicting average number of motoneurons and interneurons in sca>Nkx6 (fly gene) embryos. Scale bar: 15 µm. All images are ventral views; rostral is up, medial is left and lateral is right.