Fig. 1. Par-6 and Baz expression in the ovary. (A) Schematic of egg chambers at stages 8-10. Nurse cells (nc), and oocyte (o) are indicated. Border cells (green) are shown prior to, during and after migration. (B-M) Single confocal sections of wild-type anterior follicle cells (B-C'), and border cell clusters at the initiation of migration (E-F'), during migration (H-I'), and at the completion of migration (K-L'). Schematics of wild type stage 8 (D), early stage 9 (G), mid stage 9 (J) and stage 10 (M) egg chambers depict the position of the border cells (red) in the adjacent confocal images. Baz staining (green) is shown in panels (B,E,H,K) and double labeling with rhodamine phalloidin (red) to mark F-actin in panels (B',E',H',K'). Co-localization appears yellow. Par-6 staining (green) is shown in panels (C,F,I,L) and double labeling with phalloidin (red) in panels (C',F',I',L'). Polar cells (pc) are indicated in migrating clusters. High levels of Baz and Par-6 expression at border cell/border cell boundaries are indicated by arrows. (N-P') Three-dimensional confocal reconstruction and schematic representation of a border cell cluster in mid migration. The orientation of the border cell cluster in panel N is identical to that of the border cell cluster shown in panel H. Par-6 (green) and E-cadherin (red) are shown. Par-6 expression at the apices of the central polar cells (pc) is indicated by two straight lines.