Fig. 2. Loss of Par-6 and Baz impedes border cell migration. (A-D) Single confocal section of stage 10 egg chambers stained with anti-Singed antibody (red) or GFP (green) that are wild type (wt) (A) or contain mosaic clones for a null mutation in the baz (B-C) or the par-6 (D) locus. Homozygous mutant cells are labeled by the absence of GFP. o, Oocyte. Scale bar: 50 µm. (E) slbo-Gal4 driver, expressed in the border cells (UAS-GFP) (green), but not in the polar cells, Fascilin III (red). (F-L) Confocal images of border cell clusters that are wild type (F,H,J) or that express either two copies of RNAi par-6 in a par-6 heterozygous null background (G,K) or two copies of RNAi baz in a baz heterozygous null background (I,L). RNAi transgenes were activated using slbo-Gal4. (F-I) Merged confocal z sections of border cell clusters stained with anti-Singed antibody (red) (F-I) showing either Par-6 (green) (F,G) or Baz (green) (H,I). The position of the central polar cells (pc) is indicated by two straight lines. Scale bar: 5 µm. (J-L) Single confocal sections of stage 10 egg chambers showing Singed (green) and F-actin (red). Scale bar: 50 µm. Arrows indicate border cell cluster.