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Fig. 3. GATA6 transfected endoderm-like derivatives of ROSA11 ES cells grown as adherent cultures (A-C) and as cysts in suspension cultures (D-L) (confocal images). (A-C) During the first day of culture, the cells accumulate laminin {alpha}1 in the cytoplasm (A), which, by day 3 (B) to 4 (C), is secreted to the extracellular space. (D,G,J) Fluoresceinated phalloidin; (E,H,K) BM proteins (Cy3), see left side for antibody used; (F,I,L) merged images.