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Fig. 6. Pluripotent stem cells and their ectoderm derivatives exhibit laminin receptors. (A-D) Confocal images. (A) Day 4, 1C6 dnFgfr ES cells activated with Lamc1+/- GATA6 culture supernatant. Stained with antibody to laminin {alpha}1. The single layer of columnar cells covered by laminin {alpha}1 (red). (B) 1C6 dnFgfr ES cells incubated with Lamc1-/- GATA6 culture supernatant harvested between day four and day five of culture and stained with antibody to laminin {alpha}1. Differentiation and laminin {alpha}1 staining are both absent. (C) Wild-type ROSA11 ES cells on feeder layer stained with antibody to laminin {alpha}1 and phalloidin (green). See fibroblast in left upper quarter. There is a lack of laminin staining. (D) Wild-type ROSA11 ES cells on feeder layer treated with ROSA11-GATA6 culture supernatant for 6 hours and stained with antibody to laminin {alpha}1 (red) and phalloidin (green). There is laminin deposition in the membrane of all ES cells.