Fig. 1. Expression of the testis TAF homologs nht, rye, mia and sa. (A) Northern blot of poly(A)⁺ selected RNA probed sequentially with sequences from the protein coding regions of nht, rye and mia, and with rp49 as loading control. (B) RT-PCR products amplified using primer pairs for sa, its generally expressed Drosophila homolog dTAF8, and rp49 as loading control from Poly(A)⁺ RNA. In both A and B, RNA was from: wild-type adult males (lane 1), wild-type adult females (lane 2), adult males lacking germline (lane 3), 0- to 24-hour embryos (lane 4). (C-F) nht, rye, sa and mia mRNAs were expressed in primary spermatocytes. In situ hybridization to wild-type whole adult Drosophila testis with single stranded antisense riboprobes: (C) nht, (D) rye, (E) sa, or (F) mia. Arrows indicate the onset of mRNA expression at the start of the primary spermatocyte stage.