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Fig. 2. Loss of function of nht arrests mature primary spermatocyte differentiation and lowers expression of spermatid differentiation genes. (A,B) Phase-contrast images of whole testes dissected from (A) wild-type and (B) nht mutant males. Arrows indicate primary spermatocytes; arrowheads, elongated spermatid bundles in wild-type; the bracket in B shows the region of dying spermatocytes in nht. (C-H) In situ hybridization to whole (C,E,G) wild type or (D,F,H) nhtz-5347/Df(2L)A263 mutant testes with anti-sense mRNA probes for (C,D) cyclinB, (E,F) fuzzy onions, (G,H) don juan.