Fig. 3. Unaltered neurogenesis and altered neuronal differentiation in Lhx2^-/- embryos. In-situ hybridization analyses of OE sections with Dig-labeled cRNA probes specific for Mash1 (A), Ngn1 (B), Neurod1 (C), Golf (D), and Omp (E). Control embryos (left panel) and Lhx2^-/- embryos (right panel) show an equal distribution and number of apical and basal progenitor cells expressing Mash1 (A) and basal progenitors expressing Ngn1 (B). The OE in Lhx2^-/- embryos contains an increased number of progenitors and/or neurons that express Neurod1 (C), whereas the expression of OSN-enriched and late differentiation markers Golf (D) and Omp (E) is reduced. (F) Immunohistochemical analyses of the dorsomedial zone (dorsal left and lateral up) with a reduced number of Omp-positive cells in OE of Lhx2^-/- embryos.