Development -- Wang and Struhl 131 (21): 5367 Figure IG6

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Fig. 6. Lqf-dependent processing of Dl. Western blot analysis of Myc-tagged Dl co-overexpressed with Neur in clones of wild-type (lane 1) or lqf^- (lane 2) cells; only cells within the clones overexpress ^MycDl and Neur (see Materials and methods; the same result was obtained in all of three independent experiments). In wild-type cells, two bands are found, which correspond in size, respectively, to full-length ^MYCDl ( $~$ 105 kDa) and a truncated form ( $~$ 50 kDa) which is smaller than the size expected for the Myc-tagged Dl extracellular domain ( $~$ 75 kDa), but larger than that expected for the complementary Myc-tagged portion of Dl consisting of the transmembrane and cytosolic domains ( $~$ 40 kDa). In lqf^- cells, only the upper, apparently unprocessed, band is observed.